Composite

Part:BBa_I725100:Design

Designed by: Wayne Materi   Group: iGEM07_Alberta   (2007-10-23)


Operon for butanoate pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 308
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 763
    Illegal AgeI site found at 1077
    Illegal AgeI site found at 1208
    Illegal AgeI site found at 1988
    Illegal AgeI site found at 3091
    Illegal AgeI site found at 3493
    Illegal AgeI site found at 4717
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Each gene is preceded by its own RBS. Transformation as the part gets larger becomes more difficult and efficiencies decrease accordingly.


Source

See above

References